The University of Pennsylvania School of Nursing (more commonly referred to as Penn Nursing) is an undergraduate and graduate institution at the University of Pennsylvania, located in Philadelphia. According to U.S. News & World Report, the School of Nursing at Penn is among the top-ranked graduate nursing schools in the United States. The School of Nursing receives approximately $480 million in funding from the National Institutes of Health, making it among the most highly funded nursing schools in the country.

Penn Nursing's main building, Claire M. Fagin Hall, is located south of the center of campus. Within a block of Fagin Hall are the Hospital of the University of Pennsylvania, the Children's Hospital of Philadelphia, and buildings of the Perelman School of Medicine

The Tri-Nursing Education Building (or Tri-NEB), originally built in 1972, was later named for Claire M. Fagin, a former dean of Penn Nursing who later served as Interim President of the university. The building originally housed the Hospital of the University of Pennsylvania's School of Nursing which was a three-year diploma in nursing. Students there also attended classes at UPenn for chemistry, anatomy and physiology, and two electives of their choice. The school was founded in 1886 and graduated its last class in 1978.

When Penn accepted responsibility for HUP in 1973, HUP's school of nursing was absorbed into Penn Nursing